miRCURY LNA miRNA Power Target Site Blockers
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For studying the effects of an individual miRNA on a single target site
- Custom-designed target site blockers for specific inhibition of miRNA targets
- Sophisticated design and superior high affinity, regardless of target sequence
- Unmatched high efficacy in vitro and in vivo
- Unrivaled performance and high protein expression due to lack of RNase H-dependent mRNA degradation
- Efficient at very low concentrations, outcompeting miRNAs for their target sites
- Superior biological stability for long-lasting antisense activity
miRCURY LNA miRNA Power Target Site Blockers are antisense oligonucleotides that bind to the miRNA target site of an mRNA, preventing miRNAs from gaining access to that site. This enables you to study the effects of an miRNA on a single target. miRCURY LNA miRNA Power Target Site Blockers are efficient at very low concentrations, and due to their high affinity from LNA enhancement, the target site blockers outcompete miRNAs for their target sites.
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Cat No./ID:339194 miRCURY LNA miRNA Power Target Site Blockers (5 nmol) Go to GeneGlobe5 nmol miRCURY LNA miRNA Power Target Site Blockers with option of different labels and purifications, provided in tube format |
Cat No./ID:339195 miRCURY LNA miRNA Power Target Site Blockers (15 nmol) Go to GeneGlobe15 nmol miRCURY LNA miRNA Power Target Site Blockers with option of different labels and purifications, provided in tube format |
Cat No./ID:339199 miRCURY LNA miRNA Power Target Site Blockers, in vivo Ready (5 nmol) Go to GeneGlobe5 nmol miRCURY LNA miRNA Power Target Site Blockers, in vivo Ready, with option of different labels and purifications, provided in tube format |
Cat No./ID:339200 miRCURY LNA miRNA Power Target Site Blockers, in vivo Ready (15 nmol) Go to GeneGlobe15 nmol miRCURY LNA miRNA Power Target Site Blockers, in vivo Ready, with option of different labels and purifications, provided in tube format |
Cat No./ID:339201 miRCURY LNA miRNA Power Target Site Blockers, in vivo Large Scale Inquire miRCURY LNA miRNA Power Target Site Blockers in vivo Large Scale, purified by HPLC and Na+ salt exchange, available in amounts between 5 mg – 1 kg |
miRCURY LNA miRNA Power Target Site Blockers are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
Unravel miRNA function with target site blockers
miRNAs typically regulate gene expression of multiple targets, and inhibition of an miRNA will result in derepression of all of these targets. Therefore, a phenotype observed upon miRNA inhibition is a composite result of derepression of several targets (see figure miRNA Inhibitors vs. target site blockers). However, deregulation of a few of these targets will often contribute significantly to the phenotype. Identifying these targets is important to understanding the function of the miRNA.miRCURY LNA miRNA Power Target Site Blockers can be used to:- Determine which pathway is involved in a phenotype observed upon miRNA inhibition
- Determine which miRNA/mRNA interactions are most important in a pathway containing several predicted targets
Principle
What are target site blockers?
miRCURY LNA miRNA Target Site Blockers areLNA-enhanced antisense oligonucleotides that bind to the miRNA target site of an mRNA, thereby preventing miRNAs from gaining access to that site. This allows researchers to study the effects of an miRNA on a single target. In contrast, the phenotype observed when inhibiting an miRNA reflects the combined effects of that miRNA on all targets.LNA-enhanced target site blockers
The incorporation of LNA into the miRCURY LNA miRNA Power Target Site Blocker means that they will compete more effectively with the miRNA/RISC complex for the miRNA target site. In addition, LNA distribution throughout the LNA/DNA mixmer ensures that the antisense oligonucleotide does not catalyze RNase H-dependent degradation of the mRNA. As a result, the TSB will cause increased expression of the protein encoded by the targeted mRNA by preventing miRNA-mediated translational attenuation(see figure LNA-enhanced target site blockers compete effectively with RISC for miRNA binding site).Procedure
Following resuspension, miCURY LNA miRNA Target Site Blockers are transfected into cells with a transfection reagent or via electroporation. Phenotypic effects of thetarget site blockerare normally assessed 24–72 hours after transfection. For some applications, such as cell differentiation assays, the phenotypic readout may take place 7–10 days after transfection.
Applications
miRCURY LNA miRNA Target Site Blockers can be used for:
- Determining which pathway is involved in a phenotype observed upon miRNA inhibition
- Determining which miRNA/mRNA interactions are most important in a pathway containing several predicted targets
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