RNeasy Protect Animal Blood System
For collecting and stabilizing animal blood and purifying RNA and miRNA
- Reliable stabilization of transcript levels at blood collection
- Convenient collection and storage of small blood volumes
- Rapid purification of high-quality total RNA
- Effective recovery of miRNA
The RNeasy Protect Animal Blood System provides a complete solution for preparing high-quality RNA from animal blood. Blood from mouse, rat, and other small animals is conveniently collected in RNAprotect Animal Blood Tubes, which contain a reagent that immediately stabilizes cellular RNA. Total RNA is then purified using the RNeasy Protect Animal Blood Kit. Please note that the RNeasy Protect Animal Blood Kit and the RNAprotect Animal Blood Tubes are ordered separately.
Alternatively, the RNeasy Protect Animal Blood Kit can be used to obtain microRNA (miRNA), either in a total RNA fraction or in a separate small RNA fraction. For purification of total RNA containing miRNA, Buffer RWT (cat. no. 1067933) is also required. For purification of small RNA (containing miRNA) and large RNA in 2 separate fractions, the RNeasy MinElute Cleanup Kit (cat. no. 74204) is also required.The procedures can be fully automated on theQIAcube Connect.
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- Product Details
- Specifications
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Cat No./ID:73224 RNeasy Protect Animal Blood Kit (50) $520.00 For 50 preps: RNeasy MinElute Spin Columns, QIAshredder Spin Columns, Collections Tubes, ProteinaseK, RNase-Free DNase, and RNase-Free Reagents and Buffers; to be used with RNAprotect Animal Blood Tubes |
Cat No./ID:76544 RNAprotect Animal Blood Tubes (50x100µl) $176.00 50 tubes for collection, storage, and transport of 100µl animal blood samples with RNA stabilization; to be used with the RNeasy Protect Animal Blood Kit |
Cat No./ID:76554 RNAprotect Animal Blood Tubes (50x500µl) $316.00 50 tubes for collection, storage, and transport of 500µl animal blood samples with RNA stabilization; to be used with the RNeasy Protect Animal Blood Kit |
Product Details
Blood samples are collected in RNAprotect Animal Blood Tubes. The tubes contain a reagent that lyses blood cells and stabilizes intracellular RNA. The RNAprotect Animal Blood Tubes are centrifuged to pellet the samples, which are then washed with water and resuspended in Buffer RSB. After digestion in Buffer RBT with proteinase K, the samples are homogenized by centrifugation through QIAshredder spin columns. Ethanol is added to the samples, and the samples are then centrifuged through RNeasy MinElute spin columns. The bound total DNA is subjected to DNase digestion and washed with Buffer RW1, followed by Buffer RPE. Pure RNA is eluted in Buffer REB. The purification of RNA with the RNeasy Protect Animal Blood Kit can be fully automated on the QIAcube. A separate protocol is provided for isolation of total RNA including miRNA and requires wash Buffer RWT (ordered separately; cat. no. 1067933). For purification of small RNA (containing miRNA) and large RNA in 2 separate fractions, the RNeasy MinElute Cleanup Kit is also required.
Total RNA and miRNA purified with the RNeasy Protect Animal Blood System can be used for many downstream applications, including:
Specifications
Features | Specifications |
| Applications | Northern, dot, and slot blotting, end-point RT-PCR, quantitative, real-time RT-PCR, array analysis, next-generation sequencing |
| Elution volume | 14 µl |
| Format | Spin column |
| Main sample type | Tissue, cells |
| Processing | Manual (centrifugation) |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Total RNA |
| Sample amount | 100 µl or 500 µl animal blood |
| Technology | Silica technology |
| Yield | Rat blood (500 μl): 10–30 µg; Mouse blood (100 μl): 4–8 µg |
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