QIAamp MinElute Virus Spin Kit
For simultaneous purification of viral DNA and RNA from plasma, serum, and cell-free body fluids
- Rapid purification of high-quality viral DNA and RNA
- No organic extraction or alcohol precipitation
- Consistent, high yields
- Complete removal of contaminants and inhibitors
The QIAamp MinElute Virus Spin Kit simplifies purification of viral DNA and RNA with fast spin-column procedures. The QIAamp MinElute Virus Spin Kit uses starting sample volumes of up to 0.2 ml and combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 μl. The QIAamp MinElute Virus Spin process can be fully automated on theQIAcube Connect.
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Cat No./ID:57704 QIAamp MinElute Virus Spin Kit (50) $295.00 For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Protease, carrier RNA, buffers, Collection Tubes (2 ml) |
Product Details
Purified viral nucleic acids are free of proteins, nucleases, and other impurities, and suitable for use in sensitive downstream applications such as PCR and RT-PCR (see figures "High sensitivity in PCR" and "High sensitivity in RT-PCR").
Viral nucleic acids purified using the QIAamp MinElute Virus Spin Kit can be used in a wide range of downstream applications, including:
- PCR and quantitative real-time PCR
- Infectious disease research
The QIAamp MinElute Virus Spin Kit simplifies isolation of viral RNA and DNA from plasma, serum, and cell-free body fluids with a fast spin-column procedure. No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp MinElute silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acids to be eluted in either water or a buffer provided with the kit. QIAamp MinElute technology yields viral RNA and DNA from plasma, serum, and cell-free body fluids ready to use in PCR and blotting procedures.
The QIAamp MinElute Spin Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 µl. The procedure is suitable for use with plasma, serum, and other cell-free body fluids. QIAamp sample preparation technology is fully licensed.
The QIAamp MinElute Virus Spin Kit uses well-established technology for simultaneous purification of viral RNA and DNA from:
- Fresh or frozen plasma
- Serum
- Other cell-free body fluids
Specifications
Features | Specifications |
| Applications | PCR, real-time PCR |
| Elution volume | 20–150 µl |
| Format | MinElute columns |
| Main sample type | Serum, plasma |
| Processing | Manual (centrifugation) |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Viral DNA, viral RNA |
| Sample amount | 200 µl |
| Technology | Silica technology |
| Time per run or per prep | <1> |
| Yield | Varies |
Product Resources
Product citations in scientific articles
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