QIAamp Fast DNA Stool Mini Kit
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For isolation of gDNA from stool samples
- Streamlined, faster purification protocol
- No use of hazardous organic chemicals
- Automatable on the QIAcube
- Efficient removal of PCR inhibitors
- High sensitivity for downstream assays
The QIAamp Fast DNA Stool Mini Kit enables rapid purification of high-quality genomic DNA (human and bacterial)from fresh or frozen stool samples. The novel InhibitEX Buffer replaces cumbersome inhibitor removal tablets to efficiently remove PCR inhibitors commonly present in stool samples.Fewer protocol stepsstreamlines your workflowand reduces hands-on time during extraction. The QIAamp Fast DNA Stool Mini Kit may be used with a microcentrifuge and may be automated on theQIAcube Connect.
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Cat No./ID:19593 Inhibitex Buffer (140/250) $62.50 Inhibitex Buffer (140 ml) |
Cat No./ID:51604 QIAamp Fast DNA Stool Mini Kit (50) $285.00 For 50 DNA preps: 50 QIAamp Mini Spin Columns, QIAGEN Proteinase K, InhibitEXBuffer, wash and elution buffers, Collection Tubes (2 ml) |
The QIAamp Fast DNA Stool Mini Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
Superior yields for best downstream sensitivityThe improved protocol of the QIAamp Fast DNA Stool Mini Kit ensures highest yields from stool samples in less time than commonly used methods. Furthermore, hazardous organic chemicals are not used, improving laboratory and personal safety. Typical yields of DNA obtained with the QIAamp Fast DNA Stool Mini Kit are 5–50 µg, which is consistently higher than comparable purification kits from alternative suppliers (see figure Superior yields compared to similar purification kits). DNA purified with the QIAamp Fast DNA Stool Mini Kit gives highly sensitive results in downstream quantitative real-time PCR, with consistently lower CT values than obtained with solutions from other suppliers (see figure Higher sensitivity in qPCR). Effective removal of inhibitorsStool samples are rich in PCR inhibitors like complex polysaccharides, bile salts, lipids, and urate. In best cases, these inhibitors make targets difficult to amplify. In worst cases, their presence can entirely suppress PCR signals. Therefore, a method to completely remove such inhibitors is critical for any stool sample analysis. The novel InhibitEx Buffer of the QIAamp Fast DNA Stool Mini Kit efficiently depletes PCR inhibitors from samples, enabling reliable detection of DNA (see figure Efficient depletion of PCR inhibitors).
Principle
DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. No phenol-chloroform extraction is required. PCR inhibitors are separated from DNA by the specially formulated chemistry of the InhibitEX Buffer. Highly pure DNA ready for direct use in downstream applications is purified in as little as 25 minutes. QIAamp sample preparation technology is fully licensed.
Procedure
The QIAamp FastDNA Stool Mini Kit simplifies isolation of DNA from stool with a fast spin-column procedure (see flowchart Streamlined procedure of the QIAamp Fast DNA Stool Mini Kit) and streamlined protocols, reducing the total time needed from sample to isolated DNA to as little as 25 minutes.The unique formulation of the InhibitEX Buffer efficiently separates PCR inhibitors from DNA and, because of its liquid format, is more convenient than tablets or powder. Manual sample handling is streamlined because separate addition of inhibitor removal reagents is no longer required and experiments can be scaled more easily.The QIAamp Fast DNA Stool Mini Kit can be automated on instruments like the QIAcube.Rapid protocols are providedto efficiently isolate human DNA as well as microbial DNA for pathogen detection. Shortly, stool is suspended in InhibitEX Buffer, which separates inhibitors from DNA. Following a quick lysis, DNAbinds the QIAamp silica membrane. Any remaininginhibitorsand contaminants are removed by washing steps and pure, intact DNA is then eluted from the column.
Applications
The QIAamp Fast DNA Stool Mini Kit is for the isolation of genomicDNA (human and bacterial)from fresh or frozen stoolsamples.
Product Resources
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